Modifying Enzymes
Cayman Chemical Coenzyme A sodium salt hydra
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
New England Biolabs, Inc. Cas9 Nuclease, S. pyogenes – 400 pmol
Cas9 Nuclease, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) . The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.
TARGETMOL CHEMICALS INC AMINOPEPTIDASE M 100MG
Also available in 25 mg 50 mg 200 mg and bulk. Please contact Fisher for quotes. Aminopeptidase M (AMP) inhibition is of interest for several diseases such as highly vascularized cancer types. purity: 98%
ABclonal Technology DNA Ligase III/LIG3 Rabbit pAb
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Sigma Aldrich Fine Chemicals Biosciences Lipase from human pancreas
Pancreatic lipase fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids.
Cayman Chemical Oleoyl-L-carnItIn 5mg
A long-chain acylcarnitine and GlyT2 inhibitor (IC50 = 340 nM); selective for GlyT2 over GlyT1 (IC50 = >10,000 nM); plasma levels are increased in patients with chronic kidney disease or end-stage renal disease receiving incident hemodialysis and these are associated with cardiovascular mortality
United States Biological Corporation ZYMOLYASE 100T 50MG
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg
Sigma Aldrich Fine Chemicals Biosciences alpha Amylase from Bacillu50G
We are committed to bringing youAGreenerAAlternative Products which adhere to one or more of The 12 Principles ofAGreenerAChemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. for more information see the article in biofiles.
Gold Biotechnology Inc Proteinase K, RNase/DNase free 5 x 1 g
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Qiagen Beverly Inc T4 RNA LIGASE 2
RNA Ligase; T4 RNA Ligase 2; Conc. 30,000 U/ul; 4,500 U; incl. 10X buffer
Worthington Biochemical Corporation HYALURONIDASE PURIFIED 30KU
Chromatographically purified. A dialyzed, lyophilized powder. 37326-33-3
New England Biolabs, Inc. Exonuclease T – 250 units
Exonuclease T (Exo T), also known as RNase T, is a single-stranded RNA or DNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' to 5' direction. Exonuclease T can be used to generate blunt ends from RNA or DNA molecules that have 3 extensions.
INTACT GENOMICS INC Cas9 Nuclease 80 µg
Intact Genomics Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing (1, 2). The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining. Product Source:E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS).Product Includes: Cas9 Nuclease 10x Cas9 Nuclease Reaction Buffer
Qiagen Beverly Inc T4 POLYNUCLEOTIDE KINASE
Kinase; T4 Polynucleotide Kinase; Conc. 10,000 U/ml; 10,000 U; incl. 10X buffer
New England Biolabs, Inc. T4 RNA Ligase 2, truncated – 2000 units
T4 RNA Ligase 2' truncated (T4 Rnl2'truncated) specifically ligates the pre-adenylated 5' end of DNA or RNA to the 3 end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2'truncated is expressed from a plasmid in E. coli which encodes the first 2'9 amino acids of the full length T4 RNA Ligase 2' Unlike the full length ligase, T4 Rnl2'truncated is unable to adenylate the 5' end of the substrate, and as a result it cannot ligate the phosphorylated 5' end of RNA or DNA to the 3' end of RNA . This enzyme, also known as Rnl2'(1-2'9) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers).