Modifying Enzymes
Worthington Biochemical Corporation CATALASE 10ML
Catalase, Filtered, Minimum Activity: 40000U/mg Protein, Supplied as an aqueous solution of 2X crystallized catalase filtered through a 0.22u pore size Membrane, Store at 2 deg. to 8 deg.C, 10mL
Selleck Chemical LLC Dyngo-4a 50mg 1256493-34-1
Dyngo-4a is a potent dynamin inhibitor with IC50 of 0.38 ?M, 1.1??M, and 2.3 ?M for DynI (brain), DynI (rec), and DynII (rec), respectively. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
New England Biolabs, Inc. RNase HII – 1250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
Zymo Research Corporation Zymolyase (1000 Units Lyophilized) w/ Storage Buffer (500µl)
The primary yeast lytic activity is -1,3 glucanase and -1,3-glucan laminaripentao-hydrolase. It hydrolyzes glucose polymers at the -1,3-glucan linkages releasing laminaripentaose as the principal product.
Sigma Aldrich Fine Chemicals Biosciences Malonyl 13C3 coenzyme A li10MG
Malonyl 13C3 coenzyme A li10MG
LGC GENOMICS LLC (Lucigen) Exonuclease III, 25,000 U @ 200 U/LDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3-recessed end, producing stretches of ssDNA.
Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' endsProtect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangsThis product includes both Exonuclease III enzyme and a 10X reaction buffer.
Zymo Research Corporation DNA Degradase™ (500 U)
DNA Degradase™ is ideal for whole-genome DNA methylation analysis by a number of downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.DNA Degradase™degrades DNA to individual nucleotides.Quantitation of nucleotides to detect DNA methylation levels can be assessed by various applications (e.g.- TLC, HPLC, etc.). For detection of DNA methylation via LC/MS, DNA needs to be degraded down to individual nucleosides.
ABclonal Technology Acetyl-CoA Carboxylase
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA the rate-limiting step in fatty acid synthesis There are two ACC forms alpha and beta encoded by two different genes ACC-alpha is highly enriched in lipogenic tissues The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene
New England Biolabs, Inc. T3 DNA Ligase – 750000 units
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
New England Biolabs, Inc. α1-2,4,6 Fucosidase O - 80 units
a1-2,4,6 Fucosidase O is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal a1-2, a1-4 and a1-6 linked fucose residues from oligosaccharides. a1-2,4,6 Fucosidase O cleaves a1-6 fucose residues more efficiently than other linkages.
MINNEBIO LLC Ultra-Active Nuclease (Serratia marcescens Nuclease, EC 3.1.30.2) Benzonase Alternative Purity 98% 100 kU.
Ultra-Active Nuclease is extensively utilized across biopharmaceutical and academic research settings for its ability to streamline nucleic acid removal and improve sample processing. Beyond standard cell lysate applications, it enhances protein purification workflows by reducing nucleic acid contamination, facilitates viral vector and vaccine production by maintaining low-viscosity solutions, and supports antibody development by ensuring cleaner preparations for downstream analyses. Its broad compatibility allows use under diverse conditions, including high-salt buffers, detergents, or partially denatured samples, making it highly versatile for complex biological systems. Widely trusted by leading pharmaceutical companies, top research institutes, and university laboratories worldwide, this enzyme delivers reproducible results, contributes to higher assay accuracy, and reduces processing time in critical experimental and manufacturing workflows.
Biotium Double Stranded DNA (dsDNA)(AE-2), CF568 conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds be